Composite

Part:BBa_K5057009:Design

Designed by: Jan Zielinski   Group: iGEM24_Freiburg   (2024-09-23)


mCherry with the extracellular signal peptide HSTII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 780
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 798


Design Notes

Design consideration: We added a sequence for a 6xHis-Tag at the C-Terminus of the GOI for purification and verification of expression via Western blot.


Source

mCherry red fluorescent protein derives from the red fluorescent protein of Discosoma sp. and was described by Shaner et al. in 2004 [1]

References

[1] Shaner NC, Campbell RE, Steinbach PA, Giepmans BNG, Palmer AE, Tsien RY. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology. 2004 Nov 21;22(12):1567–72. ‌